IGF2BP1 Antibody

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ProSci
Product Code: PSI-55-194
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-194-400ul400ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing HepG2 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: RD whole cell lysate Lane 3: Karpas 299 whole cell lysate Lane 4: Raji whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: Hela whole cell lysate Lane 7: HepG2 whole cell lysate Lane 8: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
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Overlay histogram showing HepG2 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: RD whole cell lysate Lane 3: Karpas 299 whole cell lysate Lane 4: Raji whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: Hela whole cell lysate Lane 7: HepG2 whole cell lysate Lane 8: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Insulin-like growth factor 2 mRNA-binding protein 1, IGF2 mRNA-binding protein 1, IMP-1, IMP1, Coding region determinant-binding protein, CRD-BP, IGF-II mRNA-binding protein 1, VICKZ family member 1, Zipcode-binding protein 1, ZBP-1, IGF2BP1, CRDBP, VICKZ1, ZBP1
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background:
IGF2BP1 is a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Rodriguez, S., et al. Growth Horm. IGF Res. 20(4):310-318(2010)
  • Pillas, D., et al. PLoS Genet.(2), E1000856 (2010) :
  • Noubissi, F.K., et al. Cancer Res. 69(22):8572-8578(2009)
  • Zhou, Y., et al. Virology 393(2):210-220(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Zebrafish, Chicken, Xenopus
Immunogen:
This IGF2BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 508-534 amino acids from the C-terminal region of human IGF2BP1.
NCBI Gene ID #:
10642
NCBI Official Name:
Insulin-like growth factor 2 mRNA-binding protein 1
NCBI Official Symbol:
IGF2BP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
63 kDa
Protein Accession #:
Q9NZI8
Protein GI Number:
296434536
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9NZI8
User NOte:
Optimal dilutions for each application to be determined by the researcher.