RPL18A Antibody

ProSci
Product Code: PSI-63-802
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-802-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of RPL18A Antibody in 293, mouse NIH-3T3 cell line lysates (35ug/lane)
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Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of RPL18A Antibody in 293, mouse NIH-3T3 cell line lysates (35ug/lane)
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
60S ribosomal protein L18a, RPL18A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL18A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18AE family of ribosomal proteins. It is located in the cytoplasm.
Background References:
  • Wool,I.G., et.al., Biochem. Cell Biol. 73 (11-12), 933-947 (1995)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Zebrafish, Bovine
Immunogen:
This RPL18A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-166 amino acids from the C-terminal region of human RPL18A.
NCBI Gene ID #:
6142
NCBI Official Name:
60S ribosomal protein L18a
NCBI Official Symbol:
RPL18A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
Q02543
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q02543
User NOte:
Optimal dilutions for each application to be determined by the researcher.