POLDIP3 Antibody

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ProSci
Product Code: PSI-60-877
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-877-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. stomach section using POLDIP3 Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Fluorescent image of Hela cells stained with POLDIP3 Antibody (N-term). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Immunohistochemical analysis of paraffin-embedded H. stomach section using POLDIP3 Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of Hela cells stained with POLDIP3 Antibody (N-term). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Documents

Further Information

Additional Names:
Polymerase delta-interacting protein 3, 46 kDa DNA polymerase delta interaction protein, p46, S6K1 Aly/REF-like target, SKAR, POLDIP3, KIAA1649, PDIP46
Application Note:
For IHC-P starting dilution is: 1:25

For IF starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. This protein is a specific target of S6 kinase 1 and regulates cell growth. Two transcript variants that encode different protein isoforms have been identified. [provided by RefSeq].
Background References:
  • Ma, X.M., et al. Cell 133(2):303-313(2008)
  • Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
  • Smyk, A., et al. FEBS J. 273(20):4728-4741(2006)
  • Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This POLDIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of human POLDIP3.
NCBI Gene ID #:
84271
NCBI Official Name:
Polymerase delta-interacting protein 3
NCBI Official Symbol:
POLDIP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
Q9BY77
Protein GI Number:
50403796
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9BY77
User NOte:
Optimal dilutions for each application to be determined by the researcher.