IFT88 Antibody

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ProSci
Product Code: PSI-55-661
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-661-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in CEM cell line lysates (35ug/lane).
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Western blot analysis in mouse testis tissue lysates (35ug/lane).
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Confocal immunofluorescent analysis of IFT88 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in CEM cell line lysates (35ug/lane).
Western blot analysis in mouse testis tissue lysates (35ug/lane).
Confocal immunofluorescent analysis of IFT88 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10, TPR repeat protein 10, IFT88, TG737, TTC10
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq].
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Robert, A., et al. J. Cell. Sci. 120 (PT 4), 628-637 (2007) :
  • Khanna, H., et al. J. Biol. Chem. 280(39):33580-33587(2005)
  • Lehner, B., et al. Genomics 83(1):153-167(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IFT88 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 791-820 amino acids from the C-terminal region of human IFT88.
NCBI Gene ID #:
8100
NCBI Official Name:
Intraflagellar transport protein 88 homolog
NCBI Official Symbol:
IFT88
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
94 kDa
Protein Accession #:
Q13099
Protein GI Number:
206729873
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q13099
User NOte:
Optimal dilutions for each application to be determined by the researcher.