DKC1 Antibody
| Code | Size | Price |
|---|
| PSI-61-945-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
H/ACA ribonucleoprotein complex subunit 4, 5499-, CBF5 homolog, Dyskerin, Nopp140-associated protein of 57 kDa, Nucleolar protein NAP57, Nucleolar protein family A member 4, snoRNP protein DKC1, DKC1, NOLA4
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Background:
DKC1 is a member of the H/ACA snoRNPs (small
nucleolar ribonucleoproteins) gene family. snoRNPs are involved in
various aspects of rRNA processing and modification and have been
classified into two families: C/D and H/ACA. The H/ACA snoRNPs also
include the NOLA1, 2 and 3 proteins. The protein encoded by this
gene and the three NOLA proteins localize to the dense fibrillar
components of nucleoli and to coiled (Cajal) bodies in the nucleus.
Both 18S rRNA production and rRNA pseudouridylation are impaired if
any one of the four proteins is depleted. These four H/ACA snoRNP
proteins are also components of the telomerase complex. The protein
encoded by this gene is related to the Saccharomyces cerevisiae
Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in
a tail-to-tail orientation with the palmitoylated erythrocyte
membrane protein gene and is transcribed in a telomere to
centromere direction. Both nucleotide substitutions and single
trinucleotide repeat polymorphisms have been found in this gene.
Mutations in this gene cause X-linked dyskeratosis congenita, a
disease resulting in reticulate skin pigmentation, mucosal
leukoplakia, nail dystrophy, and progressive bone marrow failure in
most cases.
Background References:
- Rostamiani, K., et al. Blood Cells Mol. Dis. 44 (2), 88 (2010) :
- Grozdanov, P.N., et al. Hum. Mol. Genet. 18(23):4546-4551(2009)
- Ashbridge, B., et al. Biochemistry 48(46):10858-10865(2009)
- Sieron, P., et al. Br. J. Cancer 101(8):1410-1416(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DKC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-213 amino acids from the Central region of human DKC1.
NCBI Gene ID #:
1736
NCBI Official Name:
H/ACA ribonucleoprotein complex subunit 4
NCBI Official Symbol:
DKC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
O60832
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O60832
User NOte:
Optimal dilutions for each application to be determined by the researcher.