GTPBP2 Antibody

ProSci
Product Code: PSI-55-094
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-094-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Saccharomyces cerevisiae
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
Western blot analysis in CHO cell line lysates (35ug/lane).
2 / 5
Western blot analysis in K562 cell line lysates (35ug/lane).
3 / 5
4 / 5
GTPBP2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
5 / 5
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in CHO cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).
GTPBP2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
GTP-binding protein 2, GTPBP2 {ECO:0000312|EMBL:CAC362691}
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
GTP-binding proteins, or G proteins, constitute a superfamily capable of binding GTP or GDP. G proteins are activated by binding GTP and are inactivated by hydrolyzing GTP to GDP. This general mechanism enables G proteins to perform a wide range of biologic activities.
Background References:
  • Mungall, A.J., et al. Nature 425(6960):805-811(2003)
  • Watanabe, M., et al. Gene 256 (1-2), 51-58 (2000) :
  • Kudo, H., et al. Biochem. Biophys. Res. Commun. 272(2):456-465(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GTPBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 536-564 amino acids from the C-terminal region of human GTPBP2.
NCBI Gene ID #:
54676
NCBI Official Name:
GTP-binding protein 2
NCBI Official Symbol:
GTPBP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
Q9BX10
Protein GI Number:
74752415
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9BX10
User NOte:
Optimal dilutions for each application to be determined by the researcher.