CAMK1 Antibody

ProSci
Product Code: PSI-62-731
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-731-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody is used in Western blot to detect CAMK1 in HeLa cell lysate (lane 1) and primate brain tissue lysate (lane 2).
2 / 3
Western blot analysis of CAMK1 using rabbit polyclonal CAMK1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1 gene (Lane 2).
3 / 3

Antibody is used in Western blot to detect CAMK1 in HeLa cell lysate (lane 1) and primate brain tissue lysate (lane 2).
Western blot analysis of CAMK1 using rabbit polyclonal CAMK1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1 gene (Lane 2).

Further Information

Additional Names:
Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM-KI, CaM kinase I alpha, CaMKI-alpha, CAMK1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
Background References:
  • Condon, J.C., et al., Endocrinology 143(9):3651-3657 (2002).
  • Chin, D., et al., J. Biol. Chem. 272(50):31235-31240 (1997).
  • Haribabu, B., et al., EMBO J. 14(15):3679-3686 (1995).
  • Ishikawa, Y., et al., FEBS Lett. 550 (1-3), 57-63 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CAMK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human CAMK1.
NCBI Gene ID #:
8536
NCBI Official Name:
Calcium/calmodulin-dependent protein kinase type 1
NCBI Official Symbol:
CAMK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
Q14012
Protein GI Number:
3122301
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q14012
User NOte:
Optimal dilutions for each application to be determined by the researcher.