CTBP1 Antibody

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ProSci
Product Code: PSI-58-195
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-195-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: mouse brain lysate Lane 6: rat brain lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in Hela,HL-60,Jurkat,Raji cell line and mouse brain,rat brain tissue lysates (35ug/lane).
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Fluorescent confocal image of SK-BR-3 cell stained with CTBP1 Antibody . SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: mouse brain lysate Lane 6: rat brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela,HL-60,Jurkat,Raji cell line and mouse brain,rat brain tissue lysates (35ug/lane).
Fluorescent confocal image of SK-BR-3 cell stained with CTBP1 Antibody . SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.

Documents

Further Information

Additional Names:
C-terminal-binding protein 1, CtBP1, 111-, CTBP1, CTBP
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.
Background References:
  • Roukens, M.G., et al. Nat. Cell Biol. 12(10):933-942(2010)
  • Choi, H.J., et al. Biochem. Biophys. Res. Commun. 400(3):396-402(2010)
  • Merrill, J.C., et al. J. Mol. Biol. 398(5):657-671(2010)
  • Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CTBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-440 amino acids from the C-terminal region of human CTBP1.
NCBI Gene ID #:
1487
NCBI Official Name:
C-terminal-binding protein 1
NCBI Official Symbol:
CTBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
Q13363
Protein GI Number:
6014741
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,
Swissprot #:
Q13363
User NOte:
Optimal dilutions for each application to be determined by the researcher.