CREB1 Antibody

Product Code: PSI-56-002

Supplier: ProSci

Code	Size	Price

PSI-56-002-400ul

400ul

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit Ig

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Human
Mouse
Rat

Applications:

Fluorescence-activated cell sorting (FACS)
Immunofluorescence (IF)
Western Blot (WB)

Storage:

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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Western Blot at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: C6 whole cell lysate Lysates/proteins at 20 ug per lane.

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Western blot analysis in HT29 cell line lysates (35ug/lane).

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Documents

Further Information

Additional Names:

Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1

Application Note:

For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

Background:

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Background References:

Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Chen, G., et al. J. Neurosci. 30(39):13066-13077(2010) Serretti, A., et al.Affect Disord (2010) In pressMelnikova, V.O., et al. PLoS ONE(8), E12452 (2010) :

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted species reactivity based on immunogen sequence: Bovine

Immunogen:

This CREB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-132 amino acids from the Central region of human CREB1.

NCBI Gene ID #:

1385

NCBI Official Name:

Cyclic AMP-responsive element-binding protein 1

NCBI Official Symbol:

CREB1

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

37 kDa

Protein Accession #:

P16220

Protein GI Number:

117434

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Research Area:

Cancer,Cell Cycle,Immunology,Neuroscience,Signal Transduction

Swissprot #:

P16220

User NOte:

Optimal dilutions for each application to be determined by the researcher.