Erythropoietin Antibody

ProSci
Product Code: PSI-58-412
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-412-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western Blot at 1:2000 dilution + Rat kidney lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western Blot at 1:2000 dilution + Recombinant human EPO protein Lysates/proteins at 20 ug per lane.
3 / 4
4 / 4
Western Blot at 1:2000 dilution Lane 1: H. kidney whole cell lysate Lane 2: H. liver whole cell lysate Lane 3: M. liver whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + Rat kidney lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + Recombinant human EPO protein Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: H. kidney whole cell lysate Lane 2: H. liver whole cell lysate Lane 3: M. liver whole cell lysate Lysates/proteins at 20 ug per lane.

Further Information

Additional Names:
Erythropoietin, Epoetin, EPO
Application Note:
For WB starting dilution is: 1:2000
Background:
EPO is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq].
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Kristensen, P.L., et al. Eur. J. Endocrinol. 163(3):391-398(2010)
  • Kim, M.H., et al. Mol. Biol. (Mosk.) 44(4):656-663(2010)
  • Carraway, M.S., et al. Circ. Res. 106(11):1722-1730(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This Erythropoietin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-48 amino acids from the N-terminal region of human Erythropoietin.
NCBI Gene ID #:
2056
NCBI Official Name:
Erythropoietin
NCBI Official Symbol:
EPO
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
P01588
Protein GI Number:
119526
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P01588
User NOte:
Optimal dilutions for each application to be determined by the researcher.