CKII alpha Antibody

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ProSci
Product Code: PSI-63-424
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-424-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: C6 whole cell lysates Lane 2: MOLT-4 whole cell lysates Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma
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Western Blot at 1:2000 dilution Lane 1: C6 whole cell lysates Lane 2: MOLT-4 whole cell lysates Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma

Documents

Further Information

Additional Names:
Casein kinase II subunit alpha, CK II alpha, CSNK2A1, CK2A1
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100
Background:
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation.
Background References:
  • Miyata, Y., et al., Mol. Cell. Biol. 24(9):4065-4074 (2004).
  • Loizou, J.I., et al., Cell 117(1):17-28 (2004).
  • Filhol, O., et al., EMBO Rep. 5(4):351-355 (2004).
  • Kulartz, M., et al., Biochem. Biophys. Res. Commun. 315(4):1011-1017 (2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Rabbit, Xenopus
Immunogen:
This CKII alpha (CSNK2A1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the Central region of human CKII alpha (CSNK2A1).
NCBI Gene ID #:
1457
NCBI Official Name:
Casein kinase II subunit alpha
NCBI Official Symbol:
CSNK2A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
P68400
Protein GI Number:
55977123
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P68400
User NOte:
Optimal dilutions for each application to be determined by the researcher.