NDUFC2 Antibody

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ProSci
Product Code: PSI-61-058
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-058-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. kidney section using NDUFC2 Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Fluorescent image of HepG2 cells stained with NDUFC2 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
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Immunohistochemical analysis of paraffin-embedded R. kidney section using NDUFC2 Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
5 / 5
Western blot analysis of lysates from Hela, HepG2, U-87 MG cell line , human kidney, mouse kidney and rat kidney tissue lysate (from left to right), using NDUFC2 Antibody at 1:1000 at each lane.
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Immunohistochemical analysis of paraffin-embedded H. kidney section using NDUFC2 Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent image of HepG2 cells stained with NDUFC2 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of paraffin-embedded R. kidney section using NDUFC2 Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from Hela, HepG2, U-87 MG cell line , human kidney, mouse kidney and rat kidney tissue lysate (from left to right), using NDUFC2 Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
NADH dehydrogenase [ubiquinone] 1 subunit C2, Complex I-B145b, CI-B145b, Human lung cancer oncogene 1 protein, HLC-1, NADH-ubiquinone oxidoreductase subunit B145b, NDUFC2
Application Note:
For IHC-P starting dilution is: 1:25

For IF starting dilution is: 1:25

For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Background References:
  • Loeffen J.L.C.M.,et al.Biochem. Biophys. Res. Commun. 253:415-422(1998).
  • Dai F.Y.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
  • Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
  • Wiemann S.,et al.Genome Res. 11:422-435(2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NDUFC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-39 amino acids from the N-terminal region of human NDUFC2.
NCBI Gene ID #:
4718
NCBI Official Name:
NADH dehydrogenase [ubiquinone] 1 subunit C2
NCBI Official Symbol:
NDUFC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
14 kDa
Protein Accession #:
O95298
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
O95298
User NOte:
Optimal dilutions for each application to be determined by the researcher.