COXIV Antibody

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ProSci
Product Code: PSI-61-324
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-324-200ul200ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing NIH/3T3 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 2
Western Blot at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: BA/F3 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: C6 whole cell lysate Lysates/proteins at 20 ug per lane.
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Overlay histogram showing NIH/3T3 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: BA/F3 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: C6 whole cell lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cox4i1, Cox4, Cox4a
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background:
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Background References:
  • Grossman L.I.,et al.Nucleic Acids Res. 18:6454-6454(1990).
  • Carter R.S.,et al.Arch. Biochem. Biophys. 288:97-106(1991).
  • Carninci P.,et al.Science 309:1559-1563(2005).
  • Lubec G.,et al.Submitted (APR-2007) to UniProtKB.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This COXIV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 115-169 amino acids from the mouse region of human COXIV.
NCBI Gene ID #:
12857
NCBI Official Name:
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
NCBI Official Symbol:
Cox4i1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
P19783
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P19783
User NOte:
Optimal dilutions for each application to be determined by the researcher.