UBE2L3 Antibody

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ProSci
Product Code: PSI-60-766
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-766-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H.kidney section using UBE2L3 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Western blot analysis in K562,U87-MG,mouse NIH/3T3,rat PC-12 cell line and mouse testis,rat testis tissue lysates (35ug/lane).
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Immunohistochemical analysis of paraffin-embedded H.kidney section using UBE2L3 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis in K562,U87-MG,mouse NIH/3T3,rat PC-12 cell line and mouse testis,rat testis tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
Ubiquitin-conjugating enzyme E2 L3, L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3, UBE2L3, UBCE7, UBCH7
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.
Background References:
  • Fransen, K., et al. Hum. Mol. Genet. 19(17):3482-3488(2010)
  • Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
  • Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010)
  • Purbeck, C., et al. Biochemistry 49(7):1361-1363(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This UBE2L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the C-terminal region of human UBE2L3.
NCBI Gene ID #:
7332
NCBI Official Name:
Ubiquitin-conjugating enzyme E2 L3
NCBI Official Symbol:
UBE2L3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18 kDa
Protein Accession #:
P68036
Protein GI Number:
54039805
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
P68036
User NOte:
Optimal dilutions for each application to be determined by the researcher.