SUPV3L1 Antibody

ProSci
Product Code: PSI-64-106
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-106-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from SW620, PC-3 cell line, mouse kidney, rat kidney tissue lysate (from left to right), using SUPV3L1 Antibody at 1:1000 at each lane.
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Western blot analysis of SUPV3L1 Antibody in K562 cell line lysates (35ug/lane)
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysates from SW620, PC-3 cell line, mouse kidney, rat kidney tissue lysate (from left to right), using SUPV3L1 Antibody at 1:1000 at each lane.
Western blot analysis of SUPV3L1 Antibody in K562 cell line lysates (35ug/lane)
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
ATP-dependent RNA helicase SUPV3L1, mitochondrial, Suppressor of var1 3-like protein 1, SUV3-like protein 1, SUPV3L1, SUV3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
SUV3L1 is an ATPase and DNA/RNA helicase able to unwind DNA/DNA, DNA/RNA and RNA/RNA duplexes in the 5'-3' direction. It may protect cells from apoptosis.
Background References:
  • Szczesny, R.J., et al. Nucleic Acids Res. 38(1):279-298(2010)
  • Wang, D.D., et al. J. Biol. Chem. 284(31):20812-20821(2009)
  • Khidr, L., et al. J. Biol. Chem. 283(40):27064-27073(2008)
  • Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SUPV3L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-121 amino acids from the N-terminal region of human SUPV3L1.
NCBI Gene ID #:
6832
NCBI Official Name:
ATP-dependent RNA helicase SUPV3L1, mitochondrial
NCBI Official Symbol:
SUPV3L1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
88 kDa
Protein Accession #:
Q8IYB8
Protein GI Number:
74759699
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q8IYB8
User NOte:
Optimal dilutions for each application to be determined by the researcher.