PRS4 Antibody

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ProSci
Product Code: PSI-60-985
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-985-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
Immunohistochemical analysis of paraffin-embedded H. colon section using PRS4 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
2 / 5
Fluorescent image of Hela cells stained with PRS4 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
3 / 5
4 / 5
Fluorescent image of Hela cells stained with PRS4 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
5 / 5
Western blot analysis in A549,Hela cell line and mouse heart and lung,rat lung tissue lysates (35ug/lane).
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Immunohistochemical analysis of paraffin-embedded H. colon section using PRS4 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of Hela cells stained with PRS4 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Fluorescent image of Hela cells stained with PRS4 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis in A549,Hela cell line and mouse heart and lung,rat lung tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
26S protease regulatory subunit 4, P26s4, 26S proteasome AAA-ATPase subunit RPT2, Proteasome 26S subunit ATPase 1, PSMC1
Application Note:
For IHC-P starting dilution is: 1:25

For IF starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
Background References:
  • Dubiel W., et al. J. Biol. Chem. 267:22699-22702(1992).
  • Ebert L., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
  • Matilla A., et al. Hum. Mol. Genet. 10:2821-2831(2001).
  • Brill L.M., et al. Anal. Chem. 76:2763-2772(2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PRS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-436 amino acids from the C-terminal region of human PRS4.
NCBI Gene ID #:
5700
NCBI Official Name:
26S protease regulatory subunit 4
NCBI Official Symbol:
PSMC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
P62191
Protein GI Number:
49065817
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
P62191
User NOte:
Optimal dilutions for each application to be determined by the researcher.