CD276 Antibody
| Code | Size | Price |
|---|
| PSI-8689-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8689-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
CD276 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
CD276 Antibody: CD276 molecule, B7H3, B7-H3, B7RP-2, 4Ig-B7-H3
Application Note:
CD276 antibody can be used for Western blot at 0.5 - 1 μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.
Background References:
- Chapoval AI, Ni J, Lau JS, et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol 2001; 2:269-74.
- Sun M, Richards S, Prasad DV, et al. Characterization of mouse and human B7-H3 genes. J Immunol 2002; 168:6294-7.
- Yi KH and Chen L. Fine tuning the immune response through B7-H3 and B7-H4. Immunol Rev 2009; 229:145-51.
- Wang L, Kang FB, and Shan BE. B7-H3-mediated tumor immunology: friend or foe. Int J Cancer 2014; 134:2764-71.
Buffer:
CD276 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CD276 antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of CD276.
The immunogen is located within amino acids 484 - 534 of CD276.
The immunogen is located within amino acids 484 - 534 of CD276.
NCBI Gene ID #:
80381
NCBI Official Name:
CD276 molecule
NCBI Official Symbol:
CD276
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 35, 43, 57 kDa
Observed: 59 kDa
Observed: 59 kDa
Protein Accession #:
NP_001019907
Protein GI Number:
67188443
Purification:
CD276 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cell Cycle,Cancer,Immunology
SPECIFICITY:
At least three antibodies are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q5ZPR3
User NOte:
Optimal dilutions for each application to be determined by the researcher.