Cyclophilin B Antibody

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ProSci
Product Code: PSI-61-327
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-327-200ul200ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: BA/F3 whole cell lysate Lane 5: NIH/3T3 whole cell lysate lane 6: PC-12 whole cell lysate Lysates/proteins at 20 ug per lane.
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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: BA/F3 whole cell lysate Lane 5: NIH/3T3 whole cell lysate lane 6: PC-12 whole cell lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Peptidyl-prolyl cis-trans isomerase B, PPIase B, 5.2.1.8, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP, PPIB, CYPB
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background:
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Background References:
  • Spik G.,et al.J. Biol. Chem. 266:10735-10738(1991).
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
  • Zody M.C.,et al.Nature 440:671-675(2006).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Cyclophilin B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 161-195 amino acids from the human region of human Cyclophilin B.
NCBI Gene ID #:
5479
NCBI Official Name:
Peptidyl-prolyl cis-trans isomerase B
NCBI Official Symbol:
PPIB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
P23284
Protein GI Number:
215273869
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
P23284
User NOte:
Optimal dilutions for each application to be determined by the researcher.