GLS Antibody

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ProSci
Product Code: PSI-63-652
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-652-200ul200ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: human kidney lysate Lane 3: mouse brain lysate Lysates/proteins at 20 ug per lane.
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Fluorescent image of Hela cells stained with XAF1 GLS Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Fluorescent image of Hela cells stained with XAF1 GLS Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Western blot analysis of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using GLS Antibody at 1:1000 at each lane.
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Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: human kidney lysate Lane 3: mouse brain lysate Lysates/proteins at 20 ug per lane.
Fluorescent image of Hela cells stained with XAF1 GLS Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Fluorescent image of Hela cells stained with XAF1 GLS Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using GLS Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Glutaminase kidney isoform, mitochondrial, GLS, K-glutaminase, L-glutamine amidohydrolase, GLS, GLS1, KIAA0838
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:25

For IF starting dilution is: 1:25

For IF starting dilution is: 1:25
Background:
Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.
Background References:
  • Swierczynski,J., et.al., Biochim. Biophys. Acta 1157 (1), 55-62 (1993)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
NCBI Gene ID #:
2744
NCBI Official Name:
Glutaminase kidney isoform, mitochondrial
NCBI Official Symbol:
GLS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
73 kDa
Protein Accession #:
O94925
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Neuroscience,Signal Transduction
Swissprot #:
O94925
User NOte:
Optimal dilutions for each application to be determined by the researcher.