OAT Antibody

ProSci
Product Code: PSI-63-692
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-692-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
Western Blot at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.
2 / 5
Western blot analysis in 293,293T cell line ,mouse liver and rat liver tissue lysates (35ug/lane).
3 / 5
4 / 5
Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
5 / 5
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293,293T cell line ,mouse liver and rat liver tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Ornithine aminotransferase, mitochondrial, Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase, Ornithine aminotransferase, hepatic form, Ornithine aminotransferase, renal form, OAT
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
OAT is the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA.
Background References:
  • Michaud J., et.al., Am. J. Hum. Genet. 56:616-622(1995).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This OAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human OAT.
NCBI Gene ID #:
4942
NCBI Official Name:
Ornithine aminotransferase, mitochondrial
NCBI Official Symbol:
OAT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
P04181
Protein GI Number:
129018
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Neuroscience,Signal Transduction
Swissprot #:
P04181
User NOte:
Optimal dilutions for each application to be determined by the researcher.