PDIA6 Antibody

Contact us
ProSci
Product Code: PSI-62-385
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-385-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 7
Fluorescent image of HepG2 cells stained with XAF1 . Antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
2 / 7
Immunohistochemical analysis of paraffin-embedded H. liver section using . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
3 / 7
4 / 7
Western blot analysis of lysates from K562, HepG2, HT-1080, rat C6 cell line and human liver tissue lysate (from left to right), using at 1:1000 at each lane.
5 / 7
Western blot analysis of in Y79 cell line lysates (35ug/lane)
6 / 7
Western blot analysis of in mouse stomach tissue lysates (35ug/lane)
7 / 7
Formalin-fixed and paraffin-embedded human brain tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
❮❯

Fluorescent image of HepG2 cells stained with XAF1 . Antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H. liver section using . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from K562, HepG2, HT-1080, rat C6 cell line and human liver tissue lysate (from left to right), using at 1:1000 at each lane.
Western blot analysis of in Y79 cell line lysates (35ug/lane)
Western blot analysis of in mouse stomach tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, ER protein 5, ERp5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, PDIA6, ERP5, P5, TXNDC7
Application Note:
For IF starting dilution is: 1:100

For IHC-P starting dilution is: 1:100

For WB starting dilution is: 1:1000
Background:
Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins.
Background References:
  • Hayano,T.,Gene 164 (2), 377-378 (1995)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PDIA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-172 amino acids from the Central region of human PDIA6.
NCBI Gene ID #:
10130
NCBI Official Name:
Protein disulfide-isomerase A6
NCBI Official Symbol:
PDIA6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
Q15084
Protein GI Number:
2501205
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q15084
User NOte:
Optimal dilutions for each application to be determined by the researcher.