FUBP1 Antibody

ProSci
Product Code: PSI-57-497
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-497-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in U251,rat C6 cell line,mouse brain,rat brain tissue lysates (35ug/lane).

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in U251,rat C6 cell line,mouse brain,rat brain tissue lysates (35ug/lane).

Further Information

Additional Names:
Far upstream element-binding protein 1, FBP, FUSE-binding protein 1, DNA helicase V, hDH V, FUBP1
Application Note:
For WB starting dilution is: 1:1000-1:2000
Background:
This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.
Background References:
  • Hsiao, H.H., et al. Biochemistry 49(22):4620-4634(2010)
  • Rabenhorst, U., et al. Hepatology 50(4):1121-1129(2009)
  • Malz, M., et al. Hepatology 50(4):1130-1139(2009)
  • Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FUBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-268 amino acids from the Central region of human FUBP1.
NCBI Gene ID #:
8880
NCBI Official Name:
Far upstream element-binding protein 1
NCBI Official Symbol:
FUBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
Q96AE4
Protein GI Number:
116241370
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96AE4
User NOte:
Optimal dilutions for each application to be determined by the researcher.