RPS4X Antibody

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ProSci
Product Code: PSI-61-329
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-329-200ul200ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 2
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: rat lung lysate Lane 6: mouse lung lysate Lysates/proteins at 20 ug per lane.
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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: rat lung lysate Lane 6: mouse lung lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
40S ribosomal protein S4, X isoform, SCR10, Single copy abundant mRNA protein, RPS4X, CCG2, RPS4, SCAR
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background References:
  • Fisher E.M.C.,et al.Cell 63:1205-1218(1990).
  • Watanabe M.,et al.J. Cell Sci. 100:35-43(1991).
  • Zuo L.,et al.Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
  • Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken
Immunogen:
This RPS4X antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 209-243 amino acids from the human region of human RPS4X.
NCBI Gene ID #:
6191
NCBI Official Name:
40S ribosomal protein S4, X isoform
NCBI Official Symbol:
RPS4X
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
P62701
Protein GI Number:
50403628
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P62701
User NOte:
Optimal dilutions for each application to be determined by the researcher.