RPL34 Antibody

ProSci
Product Code: PSI-56-921
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-921-400ul400ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: U-251 MG whole cell lysate Lane 4: U-2OS whole cell lysate Lane 5: mouse brain lysate Lane 6: NIH3T3 whole cell lysate Lane 7: rat liver lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in A375 cell line lysates (35ug/lane).
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Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: U-251 MG whole cell lysate Lane 4: U-2OS whole cell lysate Lane 5: mouse brain lysate Lane 6: NIH3T3 whole cell lysate Lane 7: rat liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in A375 cell line lysates (35ug/lane).

Further Information

Additional Names:
60S ribosomal protein L34, RPL34
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Transcript variants derived from alternative splicing, alternative transcription initiation sites, and/or alternative polyadenylation exist; these variants encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Background References:
  • Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
  • Andersen, J.S., et al. Nature 433(7021):77-83(2005)
  • Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This RPL34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-66 amino acids from the Central region of human RPL34.
NCBI Gene ID #:
6164
NCBI Official Name:
60S ribosomal protein L34
NCBI Official Symbol:
RPL34
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
13 kDa
Protein Accession #:
P49207
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P49207
User NOte:
Optimal dilutions for each application to be determined by the researcher.