DMRTA2 Antibody

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ProSci
Product Code: PSI-60-806
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-806-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Zebrafish
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).
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Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.
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Western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).
Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.

Documents

Further Information

Additional Names:
Doublesex- and mab-3-related transcription factor A2, Doublesex- and mab-3-related transcription factor 5, DMRTA2, DMRT5
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
DMRTA2 may be involved in sexual development.
Background References:
  • Ottolenghi, C., et al. Genomics 79(3):333-343(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
NCBI Gene ID #:
63950
NCBI Official Name:
Doublesex- and mab-3-related transcription factor A2
NCBI Official Symbol:
DMRTA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
Q96SC8
Protein GI Number:
189044724
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction,
Swissprot #:
Q96SC8
User NOte:
Optimal dilutions for each application to be determined by the researcher.