ACTA1/alpha-actin Antibody
| Code | Size | Price |
|---|
| PSI-59-237-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Rat
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
For WB starting dilution is: 1:1000
Background:
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Background References:
- Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010)Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010) Yu, G., et al.Clin Neurosci 17(6):766-769(2010) Yu, C.H., et al. PLoS ONE(7), E11878 (2010)Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: C. Elegans, Drosophila, Xenopus, Chicken, Bovine, Mouse, Rabbit, Zebrafish, Hamster, Monkey, Pig, Yeast
Immunogen:
This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the C-terminal region of human ACTA1/Alpha-actin.
NCBI Gene ID #:
58
NCBI Official Name:
Actin, alpha skeletal muscle
NCBI Official Symbol:
ACTA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
P68133
Protein GI Number:
61218043
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P68133
User NOte:
Optimal dilutions for each application to be determined by the researcher.