PAX4 Antibody

ProSci
Product Code: PSI-62-404
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-404-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: rat liver lysates Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis of PAX4 antibody in CEM cell line lysates (35ug/lane)
3 / 4
4 / 4
Flow cytometric analysis of widr cells using PAX4 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: rat liver lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of PAX4 antibody in CEM cell line lysates (35ug/lane)
Flow cytometric analysis of widr cells using PAX4 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Paired box protein Pax-4, PAX4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
PAX4 is a member of the paired box (PAX) family of transcription factors. These proteins play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells.
Background References:
  • Hata,S., Int. J. Oncol. 33 (5), 1065-1071 (2008)
  • Zhang,Y., Diabetes Res. Clin. Pract. 81 (3), 365-369 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PAX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the Central region of human PAX4.
NCBI Gene ID #:
5078
NCBI Official Name:
Paired box protein Pax-4
NCBI Official Symbol:
PAX4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
O43316
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
O43316
User NOte:
Optimal dilutions for each application to be determined by the researcher.