GUCY2D Antibody

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ProSci
Product Code: PSI-55-919
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-919-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 4
Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane.
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4 / 4
GUCY2D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane.
GUCY2D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Retinal guanylyl cyclase 1, RETGC-1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase, ROS-GC, GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
This gene encodes a retina-specific guanylate cyclase, which is a member of the membrane guanylyl cyclase family. Like other membrane guanylyl cyclases, this enzyme has a hydrophobic amino-terminal signal sequence followed by a large extracellular domain, a single membrane spanning domain, a kinase homology domain, and a guanylyl cyclase catalytic domain. In contrast to other membrane guanylyl cyclases, this enzyme is not activated by natriuretic peptides. Mutations in this gene result in Leber congenital amaurosis and cone-rod dystrophy-6 diseases. [provided by RefSeq].
Background References:
  • Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010)
  • Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
  • Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
  • Pasadhika, S., et al. Invest. Ophthalmol. Vis. Sci. 51(5):2608-2614(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GUCY2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-570 amino acids from the Central region of human GUCY2D.
NCBI Gene ID #:
3000
NCBI Official Name:
Retinal guanylyl cyclase 1
NCBI Official Symbol:
GUCY2D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
120 kDa
Protein Accession #:
Q02846
Protein GI Number:
1345920
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q02846
User NOte:
Optimal dilutions for each application to be determined by the researcher.