ADRA1D Antibody

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ProSci
Product Code: PSI-61-046
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-046-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of MCF-7 cells using ADRA1D Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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4 / 5
Immunohistochemical analysis of paraffin-embedded R. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
5 / 5
Western blot analysis of lysates from A549, LNCaP, PC-3 cell line and rat brain tissue lysate (from left to right), using ADRA1D Antibody at 1:1000 at each lane.
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Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of MCF-7 cells using ADRA1D Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded R. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from A549, LNCaP, PC-3 cell line and rat brain tissue lysate (from left to right), using ADRA1D Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-1D adrenoceptor, Alpha-adrenergic receptor 1a, ADRA1D, ADRA1A
Application Note:
For IHC-P starting dilution is: 1:25

For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
Background References:
  • Bruno J.F.,et al.Biochem. Biophys. Res. Commun. 179:1485-1490(1991).
  • Forray C.,et al.Mol. Pharmacol. 45:703-708(1994).
  • Schwinn D.A.,et al.J. Pharmacol. Exp. Ther. 272:134-142(1995).
  • Weinberg D.H.,et al.Biochem. Biophys. Res. Commun. 201:1296-1304(1994).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Bovine, Dog, Rabbit, Sheep
Immunogen:
This ADRA1D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30amino acids from the N-terminal region of human ADRA1D.
NCBI Gene ID #:
146
NCBI Official Name:
Alpha-1D adrenergic receptor
NCBI Official Symbol:
ADRA1D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
P25100
Protein GI Number:
1168243
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction,Growth Factor
Swissprot #:
P25100
User NOte:
Optimal dilutions for each application to be determined by the researcher.