ACO2 Antibody
| Code | Size | Price |
|---|
| PSI-60-502-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Rat
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
![Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (Center). Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4°C. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.](https://www.prosci-inc.com/static-images/60-502_2.jpg "Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (Center). Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4°C. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.")
Documents
Further Information
Additional Names:
Aconitate hydratase, mitochondrial, Aconitase, Citrate hydro-lyase, ACO2
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
Background References:
- Juang, H.H., Mol. Genet. Metab. 81(3):244-252 (2004).
- Bota, D.A., et al., Nat. Cell Biol. 4(9):674-680 (2002).
- Gruer, M.J., et al., Trends Biochem. Sci. 22(1):3-6 (1997).
- Klausner, R.D., et al., Mol. Biol. Cell 4(1):1-5 (1993).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig, Bovine
Immunogen:
This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the Central region of human ACO2.
NCBI Gene ID #:
50
NCBI Official Name:
Aconitate hydratase, mitochondrial
NCBI Official Symbol:
ACO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
Q99798
Protein GI Number:
6686275
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q99798
User NOte:
Optimal dilutions for each application to be determined by the researcher.