APRT Antibody

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ProSci
Product Code: PSI-61-584
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-584-400ul400ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in MCF-7 cell line and rat kidney tissue lysates (35ug/lane).
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Western Blot at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MCF-7 cell line and rat kidney tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
Adenine phosphoribosyltransferase, APRT, APRT
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.
Background References:
  • Silva,C.H., et. al.,J. Biomol. Struct. Dyn. 25 (6), 589-597 (2008)
  • Di Pietro,V., et. al., Clin. Biochem. 40 (1-2), 73-80 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-170 amino acids from the C-terminal region of human APRT.
NCBI Gene ID #:
353
NCBI Official Name:
Adenine phosphoribosyltransferase
NCBI Official Symbol:
APRT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
P07741
Protein GI Number:
114074
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P07741
User NOte:
Optimal dilutions for each application to be determined by the researcher.