MCCC2 Antibody

ProSci
Product Code: PSI-62-540
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-540-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis in NCI-H292 cell line and rat liver tissue lysates (35ug/lane).
2 / 3
Formalin-fixed and paraffin-embedded human hepatocarcinoma with MCCC2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western blot analysis in NCI-H292 cell line and rat liver tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma with MCCC2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial, MCCase subunit beta, 3-methylcrotonyl-CoA carboxylase 2, 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta, MCCC2, MCCB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
MCCC2 is the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA.
Background References:
  • Uematsu,M., et.al., J. Hum. Genet. 52 (12), 1040-1043 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MCCC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-189 amino acids from the Central region of human MCCC2.
NCBI Gene ID #:
64087
NCBI Official Name:
Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
NCBI Official Symbol:
MCCC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
Q9HCC0
Protein GI Number:
20138731
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q9HCC0
User NOte:
Optimal dilutions for each application to be determined by the researcher.