CALR Antibody
| Code | Size | Price |
|---|
| PSI-62-527-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Rat
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, grp60, CALR, CRTC
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
Calreticulin is a multifunctional protein that acts as a
major Ca(2+)-binding (storage) protein in the lumen of the
endoplasmic reticulum. It is also found in the nucleus, suggesting
that it may have a role in transcription regulation. Calreticulin
binds to the synthetic peptide KLGFFKR, which is almost identical
to an amino acid sequence in the DNA-binding domain of the
superfamily of nuclear receptors. Calreticulin binds to antibodies
in certain sera of systemic lupus and Sjogren patients which
contain anti-Ro/SSA antibodies, it is highly conserved among
species, and it is located in the endoplasmic and sarcoplasmic
reticulum where it may bind calcium. The amino terminus of
calreticulin interacts with the DNA-binding domain of the
glucocorticoid receptor and prevents the receptor from binding to
its specific glucocorticoid response element. Calreticulin can
inhibit the binding of androgen receptor to its hormone-responsive
DNA element and can inhibit androgen receptor and retinoic acid
receptor transcriptional activities in vivo, as well as retinoic
acid-induced neuronal differentiation. Thus, calreticulin can act
as an important modulator of the regulation of gene transcription
by nuclear hormone receptors. Systemic lupus erythematosus is
associated with increased autoantibody titers against calreticulin
but calreticulin is not a Ro/SS-A antigen. Earlier papers referred
to calreticulin as an Ro/SS-A antigen but this was later disproven.
Increased autoantibody titer against human calreticulin is found in
infants with complete congenital heart block of both the IgG and
IgM classes.
Background References:
- Du, X.L., et al. Oncogene 28(42):3714-3722(2009)
- Sato, H., et al. Biochim. Biophys. Acta 1790(10):1198-1205(2009)
- Abd Alla, J., et al. Biochem. Biophys. Res. Commun. 387(1):186-190(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CALR Antibody is generated from rabbits immunized with a recombinant protein of human CALR.
NCBI Gene ID #:
811
NCBI Official Name:
Calreticulin
NCBI Official Symbol:
CALR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
P27797
Protein GI Number:
117501
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P27797
User NOte:
Optimal dilutions for each application to be determined by the researcher.