RFC5 Antibody
| Code | Size | Price |
|---|
| PSI-61-523-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Rat
Applications:
- Flow Cytometry
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Replication factor C subunit 5, Activator 1 36 kDa subunit, A1 36 kDa subunit, Activator 1 subunit 5, Replication factor C 36 kDa subunit, RF-C 36 kDa subunit, RFC36, RFC5
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.
Background References:
- Stelzl,U., Cell 122 (6), 957-968 (2005)
- Andersen,J.S., Nature 433 (7021), 77-83 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RFC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human RFC5.
NCBI Gene ID #:
5985
NCBI Official Name:
Replication factor C subunit 5
NCBI Official Symbol:
RFC5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
P40937
Protein GI Number:
728777
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P40937
User NOte:
Optimal dilutions for each application to be determined by the researcher.