KLF9 Antibody

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ProSci
Product Code: PSI-58-354
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-354-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
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Fluorescent confocal image of U251 cell stained with KLF9 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF9 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF9 immunoreactivity is localized to vesicles and Nucleus significantly.
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Western Blot at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Fluorescent confocal image of U251 cell stained with KLF9 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF9 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF9 immunoreactivity is localized to vesicles and Nucleus significantly.

Documents

Further Information

Additional Names:
Krueppel-like factor 9, Basic transcription element-binding protein 1, BTE-binding protein 1, GC-box-binding protein 1, Transcription factor BTEB1, KLF9, BTEB, BTEB1
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a transcription factor that binds to GC box elements located in the promoter. Binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription.
Background References:
  • Du, H., et al. Biol. Reprod. 83(2):205-211(2010)
  • Pabona, J.M., et al. Endocrinology 151(7):3396-3406(2010)
  • Gamper, I., et al. Exp. Hematol. 37(5):539-548(2009)
  • Kang, L., et al. Pathol. Int. 58(6):334-338(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig
Immunogen:
This KLF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human KLF9.
NCBI Gene ID #:
687
NCBI Official Name:
Krueppel-like factor 9
NCBI Official Symbol:
KLF9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
Q13886
Protein GI Number:
3913177
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q13886
User NOte:
Optimal dilutions for each application to be determined by the researcher.