PDP1 Antibody
| Code | Size | Price |
|---|
| PSI-56-435-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Rat
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial, PDP 1, Protein phosphatase 2C, Pyruvate dehydrogenase phosphatase catalytic subunit 1, PDPC 1, PDP1, PDP, PPM2C
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
Pyruvate dehydrogenase (E1) is one of the three components
(E1, E2, and E3) of the large pyruvate dehydrogenase complex.
Pyruvate dehydrogenase kinases catalyze phosphorylation of serine
residues of E1 to inactivate the E1 component and inhibit the
complex. Pyruvate dehydrogenase phosphatases catalyze the
dephosphorylation and activation of the E1 component to reverse the
effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase
phosphatase is a heterodimer consisting of catalytic and regulatory
subunits. Two catalytic subunits have been reported; one is
predominantly expressed in skeletal muscle and another one is is
much more abundant in the liver. The catalytic subunit, encoded by
this gene, is the former, and belongs to the protein phosphatase 2C
(PP2C) superfamily. Along with the pyruvate dehydrogenase complex
and pyruvate dehydrogenase kinases, this enzyme is located in the
mitochondrial matrix. Mutation in this gene causes pyruvate
dehydrogenase phosphatase deficiency. Multiple alternatively
spliced transcript variants encoding different isoforms have been
identified.
Background References:
- Kato, J., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 66 (PT 3), 342-345 (2010) :
- Cameron, J.M., et al. Hum. Genet. 125(3):319-326(2009)
- Stellingwerff, T., et al. Am. J. Physiol. Endocrinol. Metab. 290 (2), E380-E388 (2006) :
- Maj, M.C., et al. J. Clin. Endocrinol. Metab. 90(7):4101-4107(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PDP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the Central region of human PDP1.
NCBI Gene ID #:
54704
NCBI Official Name:
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
NCBI Official Symbol:
PDP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
Q9P0J1
Protein GI Number:
78099789
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9P0J1
User NOte:
Optimal dilutions for each application to be determined by the researcher.