EIF3E Antibody
| Code | Size | Price |
|---|
| PSI-60-526-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Zebrafish
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Eukaryotic translation initiation factor 3 subunit E {ECO:0000255|HAMAP-Rule:MF_03004}, eIF3e {ECO:0000255|HAMAP-Rule:MF_03004}, Eukaryotic translation initiation factor 3 subunit 6 {ECO:0000255|HAMAP-Rule:MF_03004}, Viral integration site protein INT-6 homolog, eIF-3 p48 {ECO:0000255|HAMAP-Rule:MF_03004}, EIF3E {ECO:0000255|HAMAP-Rule:MF_03004}
Application Note:
For WB starting dilution is: 1:1000
Background:
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
Background References:
- Grzmil, M., et al. Oncogene 29(28):4080-4089(2010)
- Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
- Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)
- Morris, C., et al. EMBO Rep. 8(6):596-602(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Chicken, Monkey, Mouse, Rat
Immunogen:
This EIF3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-276 amino acids from the Central region of human EIF3E.
NCBI Gene ID #:
3646
NCBI Official Name:
Eukaryotic translation initiation factor 3 subunit E {ECO:0000255|HAMAP-Rule:MF_03004}
NCBI Official Symbol:
EIF3E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
P60228
Protein GI Number:
41019126
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P60228
User NOte:
Optimal dilutions for each application to be determined by the researcher.