HSPA5 Antibody

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ProSci
Product Code: PSI-61-767
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-767-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Zebrafish
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Hela,A2058,NCI-H460 cell line lysates (35ug/lane).
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HSPA5 Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Confocal immunofluorescent analysis of HSPA5 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in Hela,A2058,NCI-H460 cell line lysates (35ug/lane).
HSPA5 Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of HSPA5 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Background:
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.
Background References:
  • Zhao, C., et al. J. Med. Virol. 82(1):14-22(2010) Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010) Arnaudeau, S., et al. Proteomics 9(23):5316-5327(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
Immunogen:
This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-289 amino acids from the Central region of human HSPA5.
NCBI Gene ID #:
3309
NCBI Official Name:
78 kDa glucose-regulated protein
NCBI Official Symbol:
HSPA5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
72 kDa
Protein Accession #:
P11021
Protein GI Number:
14916999
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P11021
User NOte:
Optimal dilutions for each application to be determined by the researcher.