DHX32 Antibody

ProSci
Product Code: PSI-57-131
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-131-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse heart tissue lysates (35ug/lane).
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DHX32 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in mouse heart tissue lysates (35ug/lane).
DHX32 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32, DEAD/H box 32, DEAD/H helicase-like protein 1, DHLP1, DEAH box protein 32, HuDDX32, DHX32, DDX32
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants, but the full length nature of one of the variants has not been defined.
Background References:
  • Huang, C., et al. J. Exp. Clin. Cancer Res. 28, 11 (2009) :
  • Chen, Y., et al. Exp. Mol. Pathol. 82(3):256-261(2007)
  • Alli, Z., et al. Anticancer Res. 27 (1A), 373-377 (2007) :
  • Alli, Z., et al. Exp. Mol. Pathol. 81(3):245-248(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DHX32 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from the Central region of human DHX32.
NCBI Gene ID #:
55760
NCBI Official Name:
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
NCBI Official Symbol:
DHX32
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
84 kDa
Protein Accession #:
Q7L7V1
Protein GI Number:
74759011
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
Q7L7V1
User NOte:
Optimal dilutions for each application to be determined by the researcher.