Myb Sumoylation Site Antibody
| Code | Size | Price |
|---|
| PSI-61-399-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Transcriptional activator Myb, Proto-oncogene c-Myb, MYB
Application Note:
For IHC-P starting dilution is: 1:50~100
For WB starting dilution is: 1:1000
For WB starting dilution is: 1:1000
Background:
Myb is a transcriptional activator; DNA-binding protein that recognizes the sequence 5'-YAAC[GT]G-3'. It participates in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed predominantly in haematopoietic cells and immature gastroepithelial cells. TRAF7 stimulates the sumoylation of Myb at Lys-523 and Lys-499, which are the same sites as those used for PIASy-induced sumoylation. A correlation has been established between reduction in sumoylation of Myb and increase in transcriptional activation. Negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on upon sumoylation.
Background References:
- Luchetti, M.M., et al., J. Biol. Chem. 278(3):1533-1541 (2003).
- Dahle, O., et al., Eur. J. Biochem. 270(6):1338-1348 (2003).
- Chen, J., et al., Oncogene 21(12):1859-1869 (2002).
- Hernandez-Munain, C., et al., J. Immunol. 169(8):4362-4369 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Myb Sumoylation Site antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from human Myb Sumoylation Site.
NCBI Gene ID #:
4602
NCBI Official Name:
Transcriptional activator Myb
NCBI Official Symbol:
MYB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
72 kDa
Protein Accession #:
P10242
Protein GI Number:
2815504
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
P10242
User NOte:
Optimal dilutions for each application to be determined by the researcher.