Mouse Cblb Antibody
| Code | Size | Price |
|---|
| PSI-60-393-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
E3 ubiquitin-protein ligase CBL-B, 632-, Casitas B-lineage lymphoma proto-oncogene b, SH3-binding protein CBL-B, Signal transduction protein CBL-B, Cblb
Application Note:
For WB starting dilution is: 1:1000
Background:
E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization.
Background References:
- Wilson, B.G., et al. Cancer Cell 18(4):316-328(2010)
- Stromnes, I.M., et al. J. Clin. Invest. 120(10):3722-3734(2010)
- Naramura, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16274-16279(2010)
- Teh, C.E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(33):14709-14714(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Cblb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 862-889 amino acids from the C-terminal region of mouse Cblb.
NCBI Gene ID #:
208650
NCBI Official Name:
E3 ubiquitin-protein ligase CBL-B
NCBI Official Symbol:
Cblb
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
109 kDa
Protein Accession #:
Q3TTA7
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Immunology
Swissprot #:
Q3TTA7
User NOte:
Optimal dilutions for each application to be determined by the researcher.