Mouse Akt1 Antibody
| Code | Size | Price |
|---|
| PSI-57-924-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
RAC-alpha serine/threonine-protein kinase, AKT1 kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, Thymoma viral proto-oncogene, Akt1, Akt, Rac
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes the founding member of the Akt
serine-threonine protein kinase gene family that also includes Akt2
and Akt3. This kinase is a major downstream effector of the
phosphatidylinositol 3-kinase (PI3K) pathway that mediates the
effects of various growth factors such as platelet-derived growth
factor (PDGF), epidermal growth factor (EGF), insulin and
insulin-like growth factor I (IGF-I). It is activated through
recruitment to cellular membranes by PI3K lipid products and by
phosphorylation by 3-phosphoinositide dependent kinase-1. It then
further phosphorylates different downstream proteins in response to
various extracellular signals and thus plays a pivotal role in
mediating a variety of cellular processes, such as glucose
metabolism, glycogen biosynthesis, protein synthesis and turn over,
inflammatory response, cell survival (anti-apoptosis) and
development. Alternatively spliced transcript variants encoding
different isoforms have been found for this gene. [provided by
RefSeq].
Background References:
- Chang, Z., et al. Dev. Biol. 347(2):384-391(2010)
- Zhou, F., et al. Am. J. Pathol. 177(4):2124-2133(2010)
- Sun, X., et al. Proc. Natl. Acad. Sci. U.S.A. 107(39):16887-16892(2010)
- Goncalves, M.D., et al. PLoS ONE(9), E12707 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Human
Immunogen:
This Mouse Akt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of mouse Akt1.
NCBI Gene ID #:
11651
NCBI Official Name:
RAC-alpha serine/threonine-protein kinase
NCBI Official Symbol:
Akt1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
P31750
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
P31750
User NOte:
Optimal dilutions for each application to be determined by the researcher.