Mouse Stk11 Antibody

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ProSci
Product Code: PSI-59-109
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-59-109-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Fluorescent image of A549 cells stained with XAF1 Mouse Stk11 Antibody . AP17316b was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
2 / 4
Immunohistochemical analysis of paraffin-embedded M.skeletal muscle section using Mouse Stk11 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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4 / 4
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
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Fluorescent image of A549 cells stained with XAF1 Mouse Stk11 Antibody . AP17316b was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded M.skeletal muscle section using Mouse Stk11 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
Serine/threonine-protein kinase STK11, Liver kinase B1 homolog, LKB1, mLKB1, Stk11 {ECO:0000312|MGI:MGI:1341870}
Application Note:
For IF starting dilution is: 1:25

For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:500-1:1000
Background:
Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases (By similarity). Tumor suppressor.
Background References:
  • Gao, Y., et al. Proc. Natl. Acad. Sci. U.S.A. 107(44):18892-18897(2010)
  • Bungard, D., et al. Science 329(5996):1201-1205(2010)
  • Koh, H.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(35):15541-15546(2010)
  • Foretz, M., et al. J. Clin. Invest. 120(7):2355-2369(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Stk11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-420 amino acids from the C-terminal region of mouse Stk11.
NCBI Gene ID #:
20869
NCBI Official Name:
Serine/threonine-protein kinase STK11
NCBI Official Symbol:
Stk11
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
Q9WTK7
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Obesity
Swissprot #:
Q9WTK7
User NOte:
Optimal dilutions for each application to be determined by the researcher.