LAMC2 Antibody
| Code | Size | Price |
|---|
| PSI-57-399-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Laminin subunit gamma-2, Cell-scattering factor 140 kDa subunit, CSF 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma, LAMC2, LAMB2T, LAMNB2
Application Note:
For WB starting dilution is: 1:1000
Background:
Laminins, a family of extracellular matrix glycoproteins,
are the major noncollagenous constituent of basement membranes.
They have been implicated in a wide variety of biological processes
including cell adhesion, differentiation, migration, signaling,
neurite outgrowth and metastasis. Laminins are composed of 3 non
identical chains: laminin alpha, beta and gamma (formerly A, B1,
and B2, respectively) and they form a cruciform structure
consisting of 3 short arms, each formed by a different chain, and a
long arm composed of all 3 chains. Each laminin chain is a
multidomain protein encoded by a distinct gene. Several isoforms
of each chain have been described. Different alpha, beta and gamma
chain isomers combine to give rise to different heterotrimeric
laminin isoforms which are designated by Arabic numerals in the
order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is
laminin 1. The biological functions of the different chains and
trimer molecules are largely unknown, but some of the chains have
been shown to differ with respect to their tissue distribution,
presumably reflecting diverse functions in vivo. This gene encodes
the gamma chain isoform laminin, gamma 2. The gamma 2 chain,
formerly thought to be a truncated version of beta chain (B2t), is
highly homologous to the gamma 1 chain; however, it lacks domain
VI, and domains V, IV and III are shorter. It is expressed in
several fetal tissues but differently from gamma 1, and is
specifically localized to epithelial cells in skin, lung and
kidney. The gamma 2 chain together with alpha 3 and beta 3 chains
constitute laminin 5 (earlier known as kalinin), which is an
integral part of the anchoring filaments that connect epithelial
cells to the underlying basement membrane. The epithelium-specific
expression of the gamma 2 chain implied its role as an epithelium
attachment molecule, and mutations in this gene have been
associated with junctional epidermolysis bullosa, a skin disease
characterized by blisters due to disruption of the epidermal-dermal
junction. Two transcript variants resulting from alternative
splicing of the 3' terminal exon, and encoding different isoforms
of gamma 2 chain, have been described. The two variants are
differentially expressed in embryonic tissues, however, the
biological significance of the two forms is not known. Transcript
variants utilizing alternative polyA_signal have also been noted in
literature.
Background References:
- Tsubota, Y., et al. Int. J. Cancer 127(9):2031-2041(2010)
- Drake, J.M., et al. J. Biol. Chem. 285(44):33940-33948(2010)
- Kariya, Y., et al. J. Biol. Chem. 285(5):3330-3340(2010)
- Zboralski, D., et al. Mol. Cancer(1), 65 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LAMC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 941-970 amino acids from the Central region of human LAMC2.
NCBI Gene ID #:
3918
NCBI Official Name:
Laminin subunit gamma-2
NCBI Official Symbol:
LAMC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
131 kDa
Protein Accession #:
Q13753
Protein GI Number:
90185107
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
Q13753
User NOte:
Optimal dilutions for each application to be determined by the researcher.