USP12 Antibody

ProSci
Product Code: PSI-58-115
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-115-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
2 / 2
Western blot analysis in A375 cell line lysates (35ug/lane).

Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Western blot analysis in A375 cell line lysates (35ug/lane).

Further Information

Additional Names:
Ubiquitin carboxyl-terminal hydrolase 12, Deubiquitinating enzyme 12, Ubiquitin thioesterase 12, Ubiquitin-hydrolyzing enzyme 1, Ubiquitin-specific-processing protease 12, USP12, UBH1, USP12L1
Application Note:
For WB starting dilution is: 1:1000
Background:
USP12is a deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.
Background References:
  • Kee, Y., et al. J. Biol. Chem. 285(15):11252-11257(2010)
  • Asano, K., et al. Nat. Genet. 41(12):1325-1329(2009)
  • Cohn, M.A., et al. J. Biol. Chem. 284(8):5343-5351(2009)
  • Dunham, A., et al. Nature 428(6982):522-528(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine
Immunogen:
This USP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-251 amino acids from the Central region of human USP12.
NCBI Gene ID #:
219333
NCBI Official Name:
Ubiquitin carboxyl-terminal hydrolase 12
NCBI Official Symbol:
USP12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
O75317
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
O75317
User NOte:
Optimal dilutions for each application to be determined by the researcher.