Mouse Hipk4 Antibody

ProSci
Product Code: PSI-57-492
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-492-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: mouse liver lysate Lane 2: mouse lung lysate Lysates/proteins at 20 ug per lane.
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Mouse Hipk4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western Blot at 1:1000 dilution Lane 1: mouse liver lysate Lane 2: mouse lung lysate Lysates/proteins at 20 ug per lane.
Mouse Hipk4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Homeodomain-interacting protein kinase 4, Hipk4, Gm162
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Protein kinase that phosphorylates murine TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter. It may act as a corepressor of transcription factors (Potential).
Background References:
  • Arai, S., et al. FEBS Lett. 581(29):5649-5657(2007)
  • Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Hipk4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 533-562 amino acids from the C-terminal region of mouse Hipk4.
NCBI Gene ID #:
233020
NCBI Official Name:
Homeodomain-interacting protein kinase 4
NCBI Official Symbol:
Hipk4
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
69 kDa
Protein Accession #:
Q3V016
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q3V016
User NOte:
Optimal dilutions for each application to be determined by the researcher.