ROPN1L Antibody

ProSci
Product Code: PSI-56-072
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-072-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse testis tissue lysates (35ug/lane).
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ROPN1L Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in mouse testis tissue lysates (35ug/lane).
ROPN1L Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Ropporin-1-like protein, ROPN1-like protein, AKAP-associated sperm protein, ROPN1L, ASP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a sperm protein, which interacts with A-kinase anchoring protein, AKAP3, through the amphipathic helix region of AKAP3. Type II regulatory subunit of cAMP-dependent protein kinase (PKARII) also binds to this helix domain of AKAP3, which allows PKARII to be targeted to specific subcellular compartments. It is suggested that sperm contains several proteins that bind to AKAPs in a manner similar to PKARII, and this encoded protein may be one of them.
Background References:
  • Carr, D.W., et al. J. Biol. Chem. 276(20):17332-17338(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey
Immunogen:
This ROPN1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-60 amino acids from the N-terminal region of human ROPN1L.
NCBI Gene ID #:
83853
NCBI Official Name:
Ropporin-1-like protein
NCBI Official Symbol:
ROPN1L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
Q96C74
Protein GI Number:
296452880
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q96C74
User NOte:
Optimal dilutions for each application to be determined by the researcher.