ABHD4 Antibody

ProSci
Product Code: PSI-57-192
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-192-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
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ABHD4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in mouse kidney tissue lysates (35ug/lane).
ABHD4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Abhydrolase domain-containing protein 4, 311-, Alpha/beta-hydrolase 4, Lyso-N-acylphosphatidylethanolamine lipase, ABHD4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (By similarity).
Background References:
  • Simon, G.M., et al. J. Biol. Chem. 281(36):26465-26472(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ABHD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-127 amino acids from the Central region of human ABHD4.
NCBI Gene ID #:
63874
NCBI Official Name:
Abhydrolase domain-containing protein 4
NCBI Official Symbol:
ABHD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
Q8TB40
Protein GI Number:
74762601
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q8TB40
User NOte:
Optimal dilutions for each application to be determined by the researcher.