IRF9 Antibody

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ProSci
Product Code: PSI-56-092
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-092-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in mouse spleen tissue lysates (35ug/lane).
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Confocal immunofluorescent analysis of IRF9 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Western Blot at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
Confocal immunofluorescent analysis of IRF9 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Interferon regulatory factor 9, IRF-9, IFN-alpha-responsive transcription factor subunit, ISGF3 p48 subunit, Interferon-stimulated gene factor 3 gamma, ISGF-3 gamma, Transcriptional regulator ISGF3 subunit gamma, IRF9, ISGF3G
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
IRF9 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Background References:
  • Maiwald, T., et al. FEBS J. 277(22):4741-4754(2010)
  • Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
  • Watanabe, T., et al. J. Clin. Invest. 120(5):1645-1662(2010)
  • Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IRF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids from the N-terminal region of human IRF9.
NCBI Gene ID #:
10379
NCBI Official Name:
Interferon regulatory factor 9
NCBI Official Symbol:
IRF9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
Q00978
Protein GI Number:
266392
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
Q00978
User NOte:
Optimal dilutions for each application to be determined by the researcher.