THEMIS Antibody

ProSci
Product Code: PSI-55-693
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-693-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse liver tissue lysates (35ug/lane).
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THEMIS Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thymoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in mouse liver tissue lysates (35ug/lane).
THEMIS Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thymoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Protein THEMIS, Thymocyte-expressed molecule involved in selection, THEMIS, C6orf190, C6orf207
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants.
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
  • Bhatti, P., et al. Radiat. Res. 173(2):214-224(2010)
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This THEMIS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 559-588 amino acids from the C-terminal region of human THEMIS.
NCBI Gene ID #:
387357
NCBI Official Name:
Protein THEMIS
NCBI Official Symbol:
THEMIS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
73 kDa
Protein Accession #:
Q8N1K5
Protein GI Number:
150421530
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
Q8N1K5
User NOte:
Optimal dilutions for each application to be determined by the researcher.